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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
04/03/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MENA, E.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
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Afiliación : |
EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCOS MONTESANO, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Soybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733 |
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DOI : |
10.3389/fpls.2019.01733 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020.
The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826). |
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Contenido : |
Abstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses. MenosAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xyl... Presentar Todo |
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Palabras claves : |
CELL WALL; DEFENSE GENES; DIAPORTHE CAULIVORA; DISEASE SYMPTOMS; INTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA); PATHOGEN COLONIZATION; SOYBEAN STEM CANKER; TRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a). |
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Thesagro : |
ENFERMEDADES DE LAS PLANTAS; PATÓGENOS; SOJA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16699/1/fpls-10-01733.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2019.01733/pdf
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Marc : |
LEADER 03822naa a2200313 a 4500
001 1060886
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2019.01733$2DOI
100 1 $aMENA, E.
245 $aSoybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020. The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826).
520 $aAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses.
650 $aENFERMEDADES DE LAS PLANTAS
650 $aPATÓGENOS
650 $aSOJA
653 $aCELL WALL
653 $aDEFENSE GENES
653 $aDIAPORTHE CAULIVORA
653 $aDISEASE SYMPTOMS
653 $aINTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA)
653 $aPATHOGEN COLONIZATION
653 $aSOYBEAN STEM CANKER
653 $aTRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a)
700 1 $aSTEWART, S.
700 1 $aMONTESANO, M.
700 1 $aPONCE DE LEÓN, I.
773 $tFrontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733
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INIA La Estanzuela (LE) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
02/12/2019 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
PEREYRA, S.; GARMENDIA, G.; CABRERA, M.; VERO, S.; PIANZZOLA, M.; DILL-MACKY, R. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Control biológico de la Fusariosis de la espiga de trigo y cebada.(Biological control of fusarium head blight of wheat and barley). |
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Fecha de publicación : |
2005 |
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Fuente / Imprenta : |
Agrociencia (Uruguay), v. 9, p. 337-343, 2005. |
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Idioma : |
Español |
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Contenido : |
SUMMARY:
Fusarium head blight is a devastating disease of wheat and barley worldwide and has been epidemic in the South America´s Southern cone for the past decade. Currently, available control measures are only partially effective and biological control could be an additional tool in an integrative approach to manage Fusarium head blight. In Uruguay, since 2003, studies have been conducted to determine the effectiveness of biocontrol agent applied to wheat spikes or to wheat residues to reduce infection or inoculum production of Gibberella zeae, respectively.Preliminary results indicate that there is a potential to biologically reduce the perithecial production of G. zeae with the application of Trichoderma harzianum to wheat residue. At this time, however, it is evident thatconsiderably more research is needed to integrate biological control into Fusarium head blight management.
RESUMEN:
La fusariosis de la espiga de trigo y cebada es una enfermedad devastadora a nivel mundial y ha sido epidémica en el Cono Sur de América del Sur en la última década. Actualmente ninguna práctica de manejo de la enfermedades por sí efectiva y el control biológico podría ser una herramienta más en el manejo integrado de la fusariosis de la espiga. En Uruguay desde 2003, se vienen llevando a cabo trabajos con el fin de determinar la efectividad del uso de agentes de biocontrol aplicados en espigas o en rastrojos de trigo en reducir la infección o la producción de inóculo primario de Gibberella zeae (Fusarium graminearum), respectivamente. Los resultados preliminares indican la potencialidad de la aplicación de Trichoderma harzianum en reducir la producción deperitecios de G. zeae en el rastrojo. Es necesario contar con mayor información para poder integrar al control biológico en el manejo de la fusariosis de la espiga. MenosSUMMARY:
Fusarium head blight is a devastating disease of wheat and barley worldwide and has been epidemic in the South America´s Southern cone for the past decade. Currently, available control measures are only partially effective and biological control could be an additional tool in an integrative approach to manage Fusarium head blight. In Uruguay, since 2003, studies have been conducted to determine the effectiveness of biocontrol agent applied to wheat spikes or to wheat residues to reduce infection or inoculum production of Gibberella zeae, respectively.Preliminary results indicate that there is a potential to biologically reduce the perithecial production of G. zeae with the application of Trichoderma harzianum to wheat residue. At this time, however, it is evident thatconsiderably more research is needed to integrate biological control into Fusarium head blight management.
RESUMEN:
La fusariosis de la espiga de trigo y cebada es una enfermedad devastadora a nivel mundial y ha sido epidémica en el Cono Sur de América del Sur en la última década. Actualmente ninguna práctica de manejo de la enfermedades por sí efectiva y el control biológico podría ser una herramienta más en el manejo integrado de la fusariosis de la espiga. En Uruguay desde 2003, se vienen llevando a cabo trabajos con el fin de determinar la efectividad del uso de agentes de biocontrol aplicados en espigas o en rastrojos de trigo en reducir la infección o la producción de inóculo primario de Gibberel... Presentar Todo |
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Palabras claves : |
BIOCONTROL; CROP RESIDUES; FUSARIUM GRAMINEARUM; FUSARIUM HEAD BLIGHT. |
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Thesagro : |
CEBADA; CONTROL BIOLÓGICO; FUSARIOSIS DE LA ESPIGA; GIBBERELLA ZEAE; TRICHODERMA; TRIGO. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02708naa a2200301 a 4500
001 1047203
005 2019-12-02
008 2005 bl uuuu u00u1 u #d
100 1 $aPEREYRA, S.
245 $aControl biológico de la Fusariosis de la espiga de trigo y cebada.(Biological control of fusarium head blight of wheat and barley).
260 $c2005
520 $aSUMMARY: Fusarium head blight is a devastating disease of wheat and barley worldwide and has been epidemic in the South America´s Southern cone for the past decade. Currently, available control measures are only partially effective and biological control could be an additional tool in an integrative approach to manage Fusarium head blight. In Uruguay, since 2003, studies have been conducted to determine the effectiveness of biocontrol agent applied to wheat spikes or to wheat residues to reduce infection or inoculum production of Gibberella zeae, respectively.Preliminary results indicate that there is a potential to biologically reduce the perithecial production of G. zeae with the application of Trichoderma harzianum to wheat residue. At this time, however, it is evident thatconsiderably more research is needed to integrate biological control into Fusarium head blight management. RESUMEN: La fusariosis de la espiga de trigo y cebada es una enfermedad devastadora a nivel mundial y ha sido epidémica en el Cono Sur de América del Sur en la última década. Actualmente ninguna práctica de manejo de la enfermedades por sí efectiva y el control biológico podría ser una herramienta más en el manejo integrado de la fusariosis de la espiga. En Uruguay desde 2003, se vienen llevando a cabo trabajos con el fin de determinar la efectividad del uso de agentes de biocontrol aplicados en espigas o en rastrojos de trigo en reducir la infección o la producción de inóculo primario de Gibberella zeae (Fusarium graminearum), respectivamente. Los resultados preliminares indican la potencialidad de la aplicación de Trichoderma harzianum en reducir la producción deperitecios de G. zeae en el rastrojo. Es necesario contar con mayor información para poder integrar al control biológico en el manejo de la fusariosis de la espiga.
650 $aCEBADA
650 $aCONTROL BIOLÓGICO
650 $aFUSARIOSIS DE LA ESPIGA
650 $aGIBBERELLA ZEAE
650 $aTRICHODERMA
650 $aTRIGO
653 $aBIOCONTROL
653 $aCROP RESIDUES
653 $aFUSARIUM GRAMINEARUM
653 $aFUSARIUM HEAD BLIGHT
700 1 $aGARMENDIA, G.
700 1 $aCABRERA, M.
700 1 $aVERO, S.
700 1 $aPIANZZOLA, M.
700 1 $aDILL-MACKY, R.
773 $tAgrociencia (Uruguay)$gv. 9, p. 337-343, 2005.
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